6IN0

Crystal structure of EphA3 in complex with 18-Crown-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	54.114, 38.181, 74.860
Unit cell angles	90.00, 100.72, 90.00

Refinement procedure

Resolution	33.280 - 1.501
R-factor	0.1965
Rwork	0.195
R-free	0.22940
RMSD bond length	0.006
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1-2155_1069: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.280	1.550
High resolution limit [Å]	1.500	1.500
Rpim	0.028	0.503
Number of reflections	48293
<I/σ(I)>	16.9
Completeness [%]	99.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	13% PEG2000 monomethyl ether, 0.1 M MES pH 6.5, 0.1 M sodium acetate