6IMW
The complex structure of endo-beta-1,2-glucanase mutant (E262Q) from Talaromyces funiculosus with beta-1,2-glucan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.169, 117.924, 93.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.640 - 2.100 |
| R-factor | 0.21098 |
| Rwork | 0.210 |
| R-free | 0.23567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6imu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS (June 1, 2017) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP (7.0.014) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.640 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.184 | 0.812 |
| Number of reflections | 60060 | 4375 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M citrate buffer (pH 4.0), 0.1M NaCl, 25%(w/v) PEG2000 |
