6IMV
The complex structure of endo-beta-1,2-glucanase from Talaromyces funiculosus with sophorose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.088, 118.104, 93.428 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.710 - 2.000 |
| R-factor | 0.18297 |
| Rwork | 0.182 |
| R-free | 0.20897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6imu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.105 |
| Data reduction software | XDS (June 1, 2017) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP (7.0.060) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.710 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.128 | 0.452 |
| Number of reflections | 69519 | 4430 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M phosphate-citrate buffer (pH 4.6), 0.1M NaCl, 20%(w/v) PEG3350 |
