6IMQ

Crystal structure of PML B1-box multimers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-10
Detector	MAR CCD 165 mm
Wavelength(s)	1.282
Spacegroup name	P 1 21 1
Unit cell lengths	36.670, 50.460, 51.430
Unit cell angles	90.00, 101.35, 90.00

Refinement procedure

Resolution	25.230 - 2.060
R-factor	0.1883
Rwork	0.186
R-free	0.22650
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.400	2.170
High resolution limit [Å]	2.060	2.060
Rmerge	0.170	0.690
Number of reflections	11287	1650
<I/σ(I)>	5.5
Completeness [%]	98.0	98.3
Redundancy	4
CC(1/2)	0.970	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG6K