6IMK
The crystal structure of AsfvLIG:CG complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.257, 55.159, 79.875 |
Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
Resolution | 29.674 - 2.502 |
R-factor | 0.2076 |
Rwork | 0.205 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6imj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.498 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.107 | 0.480 |
Number of reflections | 21031 | 2046 |
<I/σ(I)> | 17.25 | 2.67 |
Completeness [%] | 98.5 | 96.9 |
Redundancy | 5.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M BIS-TRIS pH 5.5, 10% w/v PEG 10000, 0.1 M Ammonium acetate |