6IM5

YAP-binding domain of human TEAD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2018-07-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	36.537, 89.356, 135.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.412 - 1.701
R-factor	0.1967
Rwork	0.195
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kys
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.066	0.290
Number of reflections	49632	2451
<I/σ(I)>	35.4
Completeness [%]	99.5
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	4% (v/v) 2-propanol, 100 mM sodium phosphate dibasic/citric acid (pH 4.5), and 200 mM lithium sulfate