6ILU

Endolysin LysPBC5 CBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	I 2 2 2
Unit cell lengths	81.823, 107.514, 117.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.361 - 1.601
R-factor	0.168
Rwork	0.167
R-free	0.19630
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.454
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.641
High resolution limit [Å]	1.601	1.601
Rmerge	0.061	0.337
Number of reflections	65554	5078
<I/σ(I)>	54.58
Completeness [%]	96.0
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MES, lithium sulfate