6ILS

Structure of Arabidopsis thaliana Ribokinase complexed with Ribose and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 42 21 2
Unit cell lengths	99.460, 99.460, 165.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.833 - 1.800
R-factor	0.2179
Rwork	0.216
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ilr
RMSD bond length	0.008
RMSD bond angle	1.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.101	4.303
Number of reflections	84606
<I/σ(I)>	29.6
Completeness [%]	99.8
Redundancy	13.8
CC(1/2)	0.999	0.427

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M sodium citrate (pH 5.5) , 40% (v/v) PEG 600