6ILR

Structure of Arabidopsis thaliana Ribokinase in unligand form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 42 21 2
Unit cell lengths	99.040, 99.040, 165.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.469 - 1.972
R-factor	0.2211
Rwork	0.220
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rkd
RMSD bond length	0.006
RMSD bond angle	0.923
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	1.972	1.980
Rmerge	0.100	1.788
Number of reflections	58558
<I/σ(I)>	23.8
Completeness [%]	99.8
Redundancy	13
CC(1/2)	0.999	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M sodium citrate (pH 5.5), 40 % (v/v) PEG 600