6ILF

CRYSTAL STRUCTURE OF BAT MHC CLASS I PTAL-N*01:01 FOR 2.7 ANGSTROM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	0.97892
Spacegroup name	C 2 2 21
Unit cell lengths	72.126, 87.871, 133.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.713 - 2.700
R-factor	0.219
Rwork	0.215
R-free	0.29250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vfn
RMSD bond length	0.003
RMSD bond angle	0.646
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.213	0.614
Number of reflections	12065	1349
<I/σ(I)>	2.6
Completeness [%]	75.5
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH 6.5, 25%(w/v) Polyethylene glycol 3,350