6IK9

HIV-1 reverse transcriptase with Q151M/G112S/D113A/Y115F/F116Y/F160L/I159L:DNA:dGTP ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	H 3
Unit cell lengths	284.869, 284.869, 95.892
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.741 - 2.435
R-factor	0.1884
Rwork	0.186
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xn1
RMSD bond length	0.007
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.430	2.430
Rmeas	0.075	0.830
Number of reflections	108564	17460
<I/σ(I)>	18.32	2
Completeness [%]	99.8	99.2
Redundancy	5.4
CC(1/2)	0.999	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	20 mM Bis-Tris-HCl PH 6.0, 30-40 mM di-ammonium hydrogen citrate, 20 mM MgCl2, 3.5-4% PEG 6000, 2.4% sucrose, 4.8% glycerol