6IK7
Crystal structure of tomato beta-galactosidase (TBG) 4 in complex with beta-1,3-galactobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.608, 97.954, 161.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.430 - 3.100 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.200 | 0.800 |
| Rmeas | 0.223 | 0.863 |
| Rpim | 0.083 | 0.319 |
| Number of reflections | 27531 | 1354 |
| <I/σ(I)> | 10.624 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 16% (w/v) PEG10000, 0.1M HEPES |
