6IJP
The structure of the ADAL-IMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.884, 81.288, 86.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.874 - 1.850 |
| R-factor | 0.1599 |
| Rwork | 0.159 |
| R-free | 0.17840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ada |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.077 | 0.327 |
| Number of reflections | 31541 | 4139 |
| <I/σ(I)> | 32.9 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.3 | 6.5 |
| CC(1/2) | 0.992 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 32% PEG 3350, 0.1 M NaCl, 0.1 M Tris-HCl (pH 9.0) |
