6IJM

Apo structure of the N6-methyl-AMP Deaminase from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	50.625, 79.862, 86.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.737 - 2.016
R-factor	0.1768
Rwork	0.174
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ada
RMSD bond length	0.011
RMSD bond angle	0.923
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.071	0.553
Number of reflections	23673	2287
<I/σ(I)>	20.5	2.9
Completeness [%]	98.7	98.7
Redundancy	6.4	6.4
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	26-36% PEG 3350, 0.2 M NaCl, 0.1 M HEPEs (pH 7.5)