6IIY
Crystal structure of deacetylase triple mutant (Orf2*T) that involving in teicoplanin biosynthetic pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.451, 66.671, 43.390 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.290 |
| R-factor | 0.16276 |
| Rwork | 0.162 |
| R-free | 0.18191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x9l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.382 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.340 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Number of reflections | 63373 | 6163 |
| <I/σ(I)> | 43.7 | |
| Completeness [%] | 94.7 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M imidazole malate pH8.5, 27.5 % PEG 10000 |
