6IIN

USP14 catalytic domain with IU1-248

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	77
Detector technology	CCD
Collection date	2012-11-29
Detector	RIGAKU
Wavelength(s)	0.984
Spacegroup name	P 21 21 21
Unit cell lengths	81.809, 104.351, 118.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.250 - 2.530
R-factor	0.213
Rwork	0.210
R-free	0.26400
RMSD bond length	0.003
RMSD bond angle	0.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.250	2.620
High resolution limit [Å]	2.530	2.530
Number of reflections	33924
<I/σ(I)>	16.93
Completeness [%]	98.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 3350, NH4F, Cscl, glycine