6IIL
USP14 catalytic domain bind to IU1-47
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-10 |
| Detector | RIGAKU |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.427, 81.404, 108.161 |
| Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 48528 | 3434 |
| <I/σ(I)> | 33.05 | |
| Completeness [%] | 93.7 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, NH4F, glycine, Cscl |
