6II0

Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 61
Unit cell lengths	78.640, 78.640, 450.008
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.360
R-factor	0.18517
Rwork	0.182
R-free	0.23662
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ii6
RMSD bond length	0.011
RMSD bond angle	1.436
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.360	2.360
Rmeas		0.643
Rpim		0.273
Number of reflections	60806	3219
<I/σ(I)>	6.1
Completeness [%]	97.3
Redundancy	5
CC(1/2)		0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	12% PEG 3000, 0.1M Na2HPO4/citric acid (pH 4.2), 0.4M NaCl, 10% glycerol