6IGY
Crystal structure of Aspergillus niger chitinase B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97775 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.095, 74.956, 99.195 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.818 - 1.948 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.19580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wno |
RMSD bond length | 0.004 |
RMSD bond angle | 0.610 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.948 | 1.948 |
Number of reflections | 34419 | |
<I/σ(I)> | 5.3 | |
Completeness [%] | 99.8 | |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES sodium, 800 mM potassium sodium tartrate tetrahydrate. |