6IGX
Crystal structure of human CAP-G in complex with CAP-H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 122.433, 61.998, 130.885 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.995 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.27170 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 2.995 | 2.995 |
| Rmerge | 0.069 | 0.552 |
| Rmeas | 0.082 | 0.653 |
| Number of reflections | 39494 | 6128 |
| <I/σ(I)> | 13.8 | 2.07 |
| Completeness [%] | 98.6 | 98 |
| Redundancy | 3.35 | 3.45 |
| CC(1/2) | 0.998 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, HEPES-NaOH pH 7.5, Ethylen glycol, Magnesium chloride |
