6IGW
MPZL1 mutant - S86G, V145G, Q146K,P147T,G148S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 39.991, 39.991, 193.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.955 - 1.979 |
| R-factor | 0.2413 |
| Rwork | 0.241 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oai |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.209 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.979 | 1.979 |
| Rpim | 0.028 | 0.209 |
| Number of reflections | 10881 | 561 |
| <I/σ(I)> | 20.28 | |
| Completeness [%] | 99.8 | |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20mM Tris-HCl pH 8.5, 1M Li2SO4, 1.8%(w/v) PEG8000 |
