6IGT
MPZL1 mutant - V145G, Q146K, P147T and G148S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.425, 40.417, 195.223 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 40.425 - 2.404 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oai |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.143 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.046 | 0.349 |
| Number of reflections | 23051 | 1330 |
| <I/σ(I)> | 14.89 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 50mM MES pH5.6, 100 mM (NH4)2SO4, 10mM MgCl2, 20% (w/v) PEG8000 |
