6IGA
Crystal structure of argininosuccinate lyase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 129.155, 129.155, 200.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.673 - 2.776 |
| R-factor | 0.2208 |
| Rwork | 0.219 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hy1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.942 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.776 | 2.800 |
| Rmerge | 0.142 | 0.443 |
| Number of reflections | 43973 | 2273 |
| <I/σ(I)> | 7.66 | |
| Completeness [%] | 95.8 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium tartrate dibasic, pH 7.0, 20% (w/v) polyethylene glycol 3350 |
