6IG4

Structure of mitochondrial CDP-DAG synthase Tam41, delta 74

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	173.15
Detector technology	PIXEL
Collection date	2015-09-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	109.069, 116.538, 108.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.677 - 2.261
R-factor	0.1833
Rwork	0.181
R-free	0.22010
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.126
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.260	2.260
Rmerge	0.124	0.468
Number of reflections	32658	1605
<I/σ(I)>	23.3	5.5
Completeness [%]	100.0	100
Redundancy	13	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289.15	15% Tacsimate, pH 7.0, 20 mM CdCl2, 10 mM K2Pt(CN)4