6IFX
Crystal structure of chimeric KsgA with loop 12 from Erm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.693, 79.499, 130.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.840 - 3.800 |
| R-factor | 0.30761 |
| Rwork | 0.304 |
| R-free | 0.38502 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.571 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.840 | 3.897 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 7342 | |
| <I/σ(I)> | 13.3 | 5.57 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium chloride, 0.05M Sodium cacodylate trihydrate pH 6.0, 10% w/v Polyethylene glycol 4000, 0.0005M Spermine |
