6IFW
crystal structure of chimeric construct of KsgA with loop 1 from erm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.220, 81.000, 120.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.400 - 2.950 |
| R-factor | 0.20622 |
| Rwork | 0.201 |
| R-free | 0.29418 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.588 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.026 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 15977 | |
| <I/σ(I)> | 27.76 | 4.25 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.2 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4% Tacsimate, 15% PEG 3350 |
