6IFO
Crystal structure of AcrIIA2-SpyCas9-sgRNA ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-14 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.155, 214.567, 131.971 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 49.510 - 3.313 |
| R-factor | 0.2247 |
| Rwork | 0.222 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zt0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.518 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.140 | 0.623 |
| Number of reflections | 56422 | |
| <I/σ(I)> | 5.8 | 1 |
| Completeness [%] | 98.9 | 96.8 |
| Redundancy | 5.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.1 M Bis-Tris, pH 6.0, 15% PEG 20000 |
