6IFN
Crystal structure of Type III-A CRISPR Csm complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.543, 82.334, 161.358 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 48.680 - 2.900 |
| R-factor | 0.20337 |
| Rwork | 0.201 |
| R-free | 0.24428 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ifl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.069 | 0.446 |
| Number of reflections | 70612 | 6836 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 97.2 | 94.7 |
| Redundancy | 4.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium citrate tribasic dihydrate, pH 5.0, 20% w/v polyethylene glycol 20,000. |
