6IF7
Crystal Structure of AA10 Lytic Polysaccharide Monooxygenase from Tectaria macrodonta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-08-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.221, 79.221, 68.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.020 - 2.200 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oy7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.466 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.020 | 50.020 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.065 | 0.042 | 0.261 |
| Number of reflections | 11475 | 1928 | |
| <I/σ(I)> | 16.1 | ||
| Completeness [%] | 99.0 | 99.7 | 90.6 |
| Redundancy | 4.9 | 5.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M MES PH 6.5, 1.6M Magnesium sulphate |
