6IF6
Structure of the periplasmic domain of SflA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2014-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.900, 66.900, 59.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.898 - 1.900 |
| R-factor | 0.1804 |
| Rwork | 0.176 |
| R-free | 0.22070 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.078 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.900 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.583 |
| Number of reflections | 12475 | 833 |
| <I/σ(I)> | 15.6 | 3.2 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 8.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-NaOH pH 7.5, 0.8M sodium potassium phosphate |
