6IF1

Crystal structure of Ube2K and K48-linked di-ubiquitin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	41.559, 38.792, 190.321
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	47.580 - 2.466
R-factor	0.2163
Rwork	0.215
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k9p
RMSD bond length	0.025
RMSD bond angle	2.019
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.510
High resolution limit [Å]	2.466	2.470
Number of reflections	22344
<I/σ(I)>	9.5
Completeness [%]	99.8
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	293	Tris-HCl, polyethylene glycol 3350, ammonium acetate.