6IEQ
Crystal Structure of HIV-1 Env ConM SOSIP.v7 in Complex with bNAb PGT124 and 35O22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03315 |
| Spacegroup name | P 63 |
| Unit cell lengths | 127.590, 127.590, 315.507 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 3.900 |
| R-factor | 0.24979 |
| Rwork | 0.247 |
| R-free | 0.29605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cez |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rpim | 0.040 | 0.280 |
| Number of reflections | 23583 | 1613 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 89.0 | 61.7 |
| Redundancy | 17.5 | 12.3 |
| CC(1/2) | 0.950 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.93 | 295 | 0.07 M sodium acetate, 30% (v/v) glycerol, 6% (w/v) PEG 4000, pH 3.93 |
