6IEM
Argininosuccinate lyase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 127.550, 183.820, 190.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.480 - 2.200 |
| R-factor | 0.20518 |
| Rwork | 0.203 |
| R-free | 0.24394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e9f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.327 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.480 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.215 | 1.244 |
| Number of reflections | 226389 | 32796 |
| <I/σ(I)> | 6 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 100mM Bis-Tris pH 5.5, 25% w/v polyethylene glycol 3350 |
