6IEK

Structure of RVFV Gn and human monoclonal antibody R12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 1 21 1
Unit cell lengths	97.756, 108.070, 107.418
Unit cell angles	90.00, 90.34, 90.00

Refinement procedure

Resolution	48.271 - 2.700
R-factor	0.2267
Rwork	0.225
R-free	0.25800
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	61396	6102
<I/σ(I)>	11.2
Completeness [%]	99.8	99.9
Redundancy	4.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M TRIS, PH8, 1.2M Na/K tartrate