6IE6

Crystal structure of ADCP1 tandem Agenet domain 3-4 in complex with H3K9me2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	56.891, 56.891, 210.005
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.963 - 1.700
R-factor	0.1906
Rwork	0.186
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.066	0.053	0.724
Rmeas	0.072	0.058	0.790
Rpim	0.028	0.022	0.309
Total number of observations	129592
Number of reflections	22285	1307	1096
<I/σ(I)>	10.7
Completeness [%]	95.7	94.1	97
Redundancy	5.8	6.3	6.1
CC(1/2)		0.997	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.1M HEPES pH 7.5, 2.0M Ammonium formate