6IE1

Crystal Structure of ELMO2(Engulfment and cell motility protein 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-20
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	75.182, 119.134, 169.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.331 - 2.480
R-factor	0.2074
Rwork	0.206
R-free	0.23970
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.733
Data reduction software	d*TREK
Data scaling software	HKL-3000
Phasing software	SHELXDE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.520
High resolution limit [Å]	2.480	6.730	2.480
Rmerge	0.068	0.030	0.920
Rmeas	0.071	0.032	0.960
Rpim	0.020	0.009	0.269
Number of reflections	27341	1486	1366
<I/σ(I)>	4.9
Completeness [%]	99.9	99.9	99.8
Redundancy	12.6	12.9	12.4
CC(1/2)		0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Sodium malonate pH 4.0, 12%PEG3,350