6IDP

Crystal structure of Vibrio cholerae MATE transporter VcmN in the straight form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-15
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.225, 92.027, 101.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.014 - 2.205
R-factor	0.2008
Rwork	0.198
R-free	0.24050
RMSD bond length	0.003
RMSD bond angle	0.782
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.014	46.014	2.340
High resolution limit [Å]	2.210	6.560	2.210
Rmerge	0.155	0.035	1.269
Rmeas	0.167	0.038	1.397
Total number of observations	209892
Number of reflections	29962	1275	4655
<I/σ(I)>	10.51	37	1.37
Completeness [%]	99.6	99.5	97.9
Redundancy	7.005	6.578	5.69
CC(1/2)	0.997	0.999	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	PEG500DME, Tris-HCl, magnesium formate