6ICY
Crystal structure of H7 hemagglutinin mutant H7-AGTL ( V186G, P221T) from the influenza virus A/Anhui/1/2013 (H7N9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97892 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.544, 116.544, 294.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.350 - 2.403 |
| R-factor | 0.2448 |
| Rwork | 0.244 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.668 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.350 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 30407 | |
| <I/σ(I)> | 26.39 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Sodium Chloride, 0.1M HEPES pH7.5, 1.6M Ammonium Sulfate |
