6ICT
Structure of SETD3 bound to SAH and methylated actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.976, 176.693, 125.892 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 59.872 - 1.952 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3smt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.873 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.270 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.169 | 0.868 |
| Number of reflections | 191350 | 27519 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Magnesium acetate tetrahydrate 0.1M Sodium cacodylate trihydrate pH 6.5 20% Polyethylene glycol 8000 |
