6ICM
Pseudomonas putida CBB5 NdmA with ferredoxin domain of NdmD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-30 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 118.265, 118.265, 455.692 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.460 - 2.961 |
R-factor | 0.2065 |
Rwork | 0.204 |
R-free | 0.26120 |
RMSD bond length | 0.034 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.067 |
High resolution limit [Å] | 2.950 | 2.961 |
Rmeas | 0.122 | |
Number of reflections | 40519 | |
<I/σ(I)> | 42.9 | |
Completeness [%] | 99.7 | |
Redundancy | 17.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M bicine/tris pH 8.5, 0.02M monosaccharides, 10% w/v PEG 20000, 20% v/v PEG MME 500 |