6ICI
Crystal structure of human MICAL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.211, 93.466, 71.568 |
| Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
| Resolution | 47.200 - 2.300 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4txi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 38165 | 5163 |
| <I/σ(I)> | 16.89 | 2.72 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.85 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bicine-NaOH pH 9.2, 7% MPD |
