6ICE

Crystal structure of Hamster MIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	53.877, 55.846, 112.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.850 - 1.800
R-factor	0.17751
Rwork	0.176
R-free	0.21284
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mff
RMSD bond length	0.020
RMSD bond angle	1.807
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.080	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.103	0.525
Rmeas	0.119	0.605
Rpim	0.059	0.297
Number of reflections	31590	1840
<I/σ(I)>	11.8
Completeness [%]	98.5	99.7
Redundancy	7.6	7.8
CC(1/2)	0.997	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	200 mM Succinic Acid (pH 7.0), 20% PEG3350