6ICA

The crystal structure of Legionella pneumophila LapA aminopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97876
Spacegroup name	P 21 21 21
Unit cell lengths	84.424, 121.913, 130.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.518 - 2.195
R-factor	0.1453
Rwork	0.143
R-free	0.18340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gne
RMSD bond length	0.009
RMSD bond angle	1.136
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.195	2.200
Rpim	0.052	0.247
Number of reflections	69504	3400
<I/σ(I)>	13
Completeness [%]	99.8
Redundancy	5.3
CC(1/2)	0.995	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.2 M Ammonium sulfate, 20% PEG3350