6IC9
Crystal structure of the SPOC domain of human PHF3 in complex with RNA polymerase II CTD diheptapeptide phosphorylated on Ser2Ser7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.215, 68.106, 100.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.100 - 1.748 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3150) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 1.780 |
| High resolution limit [Å] | 1.748 | 1.750 |
| Rmerge | 0.058 | 2.753 |
| Rmeas | 0.066 | 3.143 |
| Rpim | 0.030 | 1.476 |
| Number of reflections | 39627 | 1968 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.2 | 91.3 |
| Redundancy | 4.4 | 4.1 |
| CC(1/2) | 0.999 | 0.223 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1 M HEPES pH 7.0, 10% PEG 6000 |
