6IC8

Crystal structure of the SPOC domain of human PHF3 in complex with RNA polymerase II CTD diheptapeptide phosphorylated on Ser2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-16
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97890
Spacegroup name	P 21 21 21
Unit cell lengths	54.069, 68.019, 99.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.471 - 1.929
R-factor	0.1861
Rwork	0.184
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.949
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_3150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.471	1.998
High resolution limit [Å]	1.929	1.929
Rmerge	0.072	1.305
Rmeas	0.078	1.444
Rpim	0.031	0.603
Number of reflections	27007	1970
<I/σ(I)>	12.9	0.96
Completeness [%]	95.6	70.6
Redundancy	6.2	5.2
CC(1/2)	0.999	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.12 M ethylene glycol mixture, 0.1 M Tris-Bicine buffer pH 8.5, 20% glycerol, 10% PEG4000