6IBO
Catalytic deficiency of O-GlcNAc transferase leads to X-linked intellectual disability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99187 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 137.737, 150.617, 199.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.170 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.25368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c1d |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.781 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.230 |
| High resolution limit [Å] | 1.500 | 2.170 |
| Number of reflections | 286887 | 23302 |
| <I/σ(I)> | 11.3 | 1.3 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 5.2 | 5.3 |
| CC(1/2) | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.45 M K2HPO4, 8 mM EDTA and 1% xylitol |
