6IAZ

The archaeal Methanocaldococcus infernus Elp3 with N-terminus deletion (1-46)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	63.350, 63.350, 250.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.818 - 1.901
R-factor	0.1958
Rwork	0.195
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l7l
RMSD bond length	0.007
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.900	1.900
Rmeas	0.068	0.997
Number of reflections	41281
<I/σ(I)>	19.34
Completeness [%]	99.8
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M citrate (pH 3.8), 0.1 M LiCl and 17% PEG6000