6IAS
structure of human NKp46 in complex with antibody NKp46-1 and NKp46-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.657, 61.101, 84.138 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 36.760 - 1.750 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.250 | 1.820 |
| High resolution limit [Å] | 1.725 | 1.725 |
| Number of reflections | 50086 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MMT pH8.5-9.5, 20-30%(w/v) PEG 1500 |
