6IAJ

Sixty minutes iron loaded Rana Catesbeiana H' ferritin variant H54N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95372
Spacegroup name	F 4 3 2
Unit cell lengths	184.130, 184.130, 184.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.980 - 1.620
R-factor	0.15525
Rwork	0.154
R-free	0.18368
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lqh
RMSD bond length	0.009
RMSD bond angle	1.549
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.600	1.710
High resolution limit [Å]	1.620	1.620
Rmerge	0.115	0.390
Rmeas	0.130	0.463
Rpim	0.045	0.180
Number of reflections	34490	4928
<I/σ(I)>	10.5	3.6
Completeness [%]	100.0	100
Redundancy	7.8	6.4
CC(1/2)	0.995	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	281	1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0