6IA7

T. brucei IFT22 GTP-bound crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 61
Unit cell lengths	55.845, 55.845, 263.443
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.363 - 2.300
R-factor	0.2167
Rwork	0.212
R-free	0.26390
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.102	2.088
Number of reflections	20689	1996
<I/σ(I)>	16.7	0.9
Completeness [%]	99.7	96.7
Redundancy	9.9	8.8
CC(1/2)	0.999	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	20% (w/v) PEG3350, 50 mM NaCacodylate pH 6.5 and 200 mM calcium acetate